Figure 4.
pS6-IR in the medial basal hypothalamus of mouse models with specific alterations in LepRb signaling. A, Ad libitum-fed (Ad Lib Fed) wild-type (+/+), db/db, s/s, and l/l mice were perfused and processed for the immunofluorescent detection of pS6-IR. Representative images of pS6-IR from each genotype are shown. B, Ad libitum-fed and 24-h-fasted l/l mice were treated with leptin (Lep) or PBS, as indicated, perfused, and processed for the immunofluorescent detection of pS6-IR. Representative images are shown for each panel. Veh, vehicle; 3V, third ventricle.