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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: J Biol Chem. 2002 Jun 4;277(33):30198–30207. doi: 10.1074/jbc.M204736200

Fig. 6. Specific phosphatidylinositol 3-phosphate (PtdIns(3)P) recognition by the Cvt20 PX domain.

Fig. 6

A, protein-lipid overlay assay. Serial dilutions of phosphoinositides were spotted onto nitrocellulose membrane and incubated with purified GST-Cvt13 PX or GST-Cvt20 PX domain fusion protein. The membrane was washed and analyzed by immunoblot with antibodies to GST. B, Coomassie-stained gel of purified GST-Cvt13 PX and GST-Cvt20 PX domain fusion protein. C, PtdIns(3)P-dependent localization of Cvt13-GFP and GFP-Cvt20 by fluorescence microscopy. Cells from vps34Δ and fab1Δ strains expressing either Cvt13-GFP or GFP-Cvt20 were grown in SMD and visualized by fluorescence microscopy.