Figure 2.
Effect of 1α,25(OH)2D3 on matrix vesicle dependent activation of TGF-β1 and TGF-β2 from recombinant simian TGF-β1 and recombinant simian TGF-β2, respectively. Matrix vesicles were isolated from cultures of rat costochondral growth zone chondrocytes and incubated directly with latent growth factor in the presence of 10−9 M 1α,25(OH)2D3. Active TGF-β in the samples was measured by ELISA specific for the active form of the growth factor.
