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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Dev Biol. 2009 Jul 23;334(1):97–108. doi: 10.1016/j.ydbio.2009.07.021

Figure 3.

Figure 3

Figure 3

Immunofluorescent staining for β-catenin (A to D) in E14 control (C & D) and CatnbEx3(cko/+) (A & B) main-stem bronchi (MSB) and lungs. Panels A, C and E are frontal plans of MSB. Doted lines in panels A, C and E outline the airway of MSBs. Note β-catenin is present in both sides of MSB in the wild type lung (Green in C), but β-catenin accumulates preferentially in the outer wall epithelium of the mutant MSB (Bright Green in A). Panels E & F show LacZ staining in Nkx2.1-cre; R26R-LacZ MSB and lung, respectively.

Immunofluorescent staining for Pten in E14 control (G to I) and Nkx2.1-cre; Pten(f/f) [Pten(Δ/Δ), J to L] MSB and distal lungs. Frontal plans of MSB are shown. Doted lines indicate the adjunction between epithelium and mesenchyme of MSB (G, H, J, and K) and distal lung (I and L). Note Pten is uniformly expressed in the epithelial cells of control MSB and distal lung but is not detectable in epithelial cells of Nkx2.1-cre; Pten(f/f) MSB (J & K) and distal lung (L). Panels G & J were counterstained with DAPI. Scale bar: 80 um.