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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: Science. 2008 May 23;320(5879):1050–1054. doi: 10.1126/science.1158265

Fig. 2.

Fig. 2

The Trx system is a major SNO–caspase-3 denitrosylating activity. Data are presented as mean ± SEM; n = 3. (A) Caspase-3 activity was determined (with Z-DEVD-AMC) after a 30-min incubation of SNO–caspase-3 (∼100 nM) with a cytosolic fraction prepared from untreated HeLa cells or from cells that were transfected for 3 days with siRNA for Trx1. (B) Caspase-3 activity was determined after a 30-min incubation of SNO–caspase-3 with HeLa cytosolic extract or cytosol that had been depleted of Trx1 or TRP14 by using specific antibodies against Trx1 or TRP14. (C) Caspase-3 activity was determined after a 30-min incubation of SNO–caspase-3 (∼100 nM) with NADPH (100 μM) and recombinant human Trx1 (10 nM) and/or recombinant rat TrxR1 (10 nM). (D) Caspase-3 activity after a 30-min incubation with recombinant Escherichia coli Trx1.