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. Author manuscript; available in PMC: 2010 Oct 23.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Aug 11;388(3):533–538. doi: 10.1016/j.bbrc.2009.08.038

Figure 3. mRNA is associated with ACVs.

Figure 3

Total RNA isolated from primary chondrocytes or ACVs was reverse transcribed using random hexamers for priming, and RT reactions were subjected to PCR with primers to the indicated genes. RT-PCR products were then resolved on an agarose gel. Water was used as a negative control. For each gene set, lane M contains size markers (100-, 600-, and 1500-bp bands are indicated), lane 1 contains RT-PCR products from chondrocyte RNA, lane 2 contains RT-PCR products from ACVs, and lane 3 shows the products of a PCR reaction using water.