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RICK kinase activity is not impaired by psoralen/UV treatment. RICKwtdCst was purified from control lysate or lysate treated for 30 min with psoralen/UV. Equal protein quantities were resolved on 10% acrylamide SDS gel and stained for total protein (Coomassie blue, A). In parallel, samples were incubated with [γ-33P]ATP under kinase appropriate conditions (see Materials and Methods) and the radioactivity incorporated into a histone H2b substrate was measured (B).
