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Sleep restriction apparatus. To sleep restrict mice, a 6 h cycle composed of 4 h of enforced waking and 2 h of uncontrolled behavioral state (4 h on and 2 h off) was used. Mice were placed in a Plexiglas cage with no bottom, ¼ inch above a treadmill that moved at a speed of 3 cm/s. Food and water tubes were placed in each cage.
