Figure 4. inhibitory oligodeoxynucleotide incubated PDC induce high T cell proliferation.
After 3 days of co-culture with differentially treated PDC, T cell proliferation was measured by MLR. PDC were co-cultured with T cells at PDC/T cell ratios of 1:10, 1:100 and 1:1000. The incorporation of (3H)thymidine of proliferating T cells was indicated as counts per minute (cpm) on the y-axis. Average data from 6 independent experiments were shown. Error bars indicate SEM. (*p<0.05) (A). Mean of cpm values ± SEM of unstimulated PDC (Con), PDC incubated with iODN 2088 with GGGG-motif, iODN with TTAGGGG motif and control ODN (n=3, every experiment was triplicate) is shown, APC/T-cell ratio is depicted on the x-axis (B).