Skip to main content
. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: Nature. 2009 Jul 29;460(7259):1154–1158. doi: 10.1038/nature08262

Figure 2. C/EBP-β is required for the initiation of the myoblast to brown fat conversion through PRDM16.

Figure 2

a, Western blot analysis for C/EBP-β and PRDM16 in C2C12 myoblasts expressing scr or sh-C/EBP-β with PRDM16 or vector. b, Pparg2 gene expression. n=3. c, These cells were stained with Oil-Red-O 6 days after inducing adipocyte differentiation. d, BAT-selective gene expression. n=4. e, Microarray analysis of undifferentiated C2C12 myoblasts expressing scr or sh-C/EBP-β with PRDM16 or vector. n=3. f, Top: H&E staining of BAT from WT and C/EBP-β KO mice. Bottom: Immunohistochemistry to detect UCP1 expression. Scale bar, 20 µm. g, mRNA expression of BAT and skeletal-muscle-selective genes in BAT from E17.5 embryos. n=5–8; error bars are s.e.m.; *P<0.05, **P<0.01.