Figure 3.

Improved ligation yield with special adapters. The autoradiogram shows the bridge-assisted ligation of primer binding sites to a ³²P-labeled in vitro transcribed RNA library (mixture of 50 and 51mer due to the partial non-templated addition of a single nucleotide) (1). While ligation to the RNA’s 5′ end with the forward adapter (plus 25 nt) was acceptable (2), ligation to the 3′ end with reverse adapter 1 only (plus 18 nt) (3) and the overall ligation yield (plus 43 nt) (4) were insufficient, because N + 1 transcripts were not ligated. By using an equimolar mixture of reverse adapters 1 and 2, the 3′ ligation efficiency (5) and the overall ligation yield (6) were markedly improved.