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. Author manuscript; available in PMC: 2010 Sep 24.
Published in final edited form as: J Phys Chem B. 2009 Sep 24;113(38):12773–12781. doi: 10.1021/jp903554n

Figure 5.

Figure 5

End-products derived from the combination of dG(-H)• and •CH3 radicals. Deoxygenated 5 mM phosphate buffer solutions (1 ml) containing 0.1 mM dG (0.1 μmol), 0.1 mM dimethyl sulfoxide (0.1 μmol) and 10 mM Na2S2O8 were illuminated with 300 – 340 nm steady-state irradiation (~100 mW/cm2) from a 100 W Xe arc lamp for 15 s. Reversed-phase HPLC elution conditions (detection of products at 260 nm): 1 – 20% gradient of acetonitrile in 20 mM ammonium acetate over 60 min. The unmodified dG elutes at 11.3 min, and 8-Me-dG and N2-Me-dG elute at 17.8 (1) and 19.0 (2) min, respectively. The S2O82− anions elute near the void volume at 1.5 min. The inset shows the dependence of the Me-dG yields on irradiation time. The product yields were estimated by integrating the areas under each elution band in the HPLC profiles.