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Predicted response to cooling for rat (A) and mouse (B) sperm. Predictions are based on rat sperm in egg yolk media III and mouse sperm in low-CPA raffinose glycerol media. The model-simulated dynamic cooling response using the combined-fit Lpg and ELp values were tested for various cooling rates. The nondimensional volume is plotted along the y-axis and the subzero temperatures are shown along the x-axis. The optimal cooling rate range is shown as the shaded area.
