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Recovered motility of (A) rat and (B) mouse sperm after nucleation and freezing at various rates to −80°C and thawing at 130°C/min. Rat sperm were frozen in egg yolk media III and mouse sperm in low-CPA raffinose glycerol media. Values are given as means ± SD. The optimal cooling range from Figure 3 is also shown as the shaded area for comparison.
