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. 2009 Jun 24;81(4):739–748. doi: 10.1095/biolreprod.109.076497

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© 2009 by the Society for the Study of Reproduction, Inc.

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FIG. 4. — A) Immunoprecipitation (IP) from the nuclear fraction of mouse spermatogenic cells with anti-NASP antibody precipitated tNASP (I, lane 1), and coimmunoprecipitated HSPA2 (I, lane 2) and CDC2 (II, lane 1) but not cyclin B1 (II, lane 2). B) Immunoprecipitation from the nuclear fraction of mouse spermatogenic cells with anti-cyclin B1 antibody precipitated cyclin B1 (I, lane 1) and CDC2 (I, lane 2) but not HSPA2 (II, lane 1) or tNASP (II, lane 2). C) PhosphorImager detection of ³²P-labeled histone indicating specific kinase activity of CDC2 containing immunoprecipitates. Immunoprecipitation with anti-NASP antibody does not have any kinase activity in the precipitate (lane 1); however, when the immunoprecipitation is done with anti-cyclin B1 antibody (lane 2), a strongly radioactive H1 band demonstrates specific kinase activity in the precipitate. Control panel demonstrates equal amounts of antibodies (IgG) used.