FIG. 5.
Invasive index of ADM2-treated HTR-8SV/neo cells. Cells were cultured on Matrigel-coated transwell filters and treated with or without ADM2 (10−8–10−9 M) in the presence or absence of ADM2(17–47) 10−6 M, ADM2(17–47) 10−5 M, ADM2(17–47) 10−4 M, or ADM2(17–47) 10−5 M for 48 h. Invasive cells that penetrated to the downward surface of the insert were incubated with MTT, and the optical absorbance at 550 nm was measured using a microplate reader. The invasive index was calculated as the ratio of the percentage of invasion in ADM2-treated cells to the percentage of invasion of control nontreated cells. Data are expressed as the mean ± SEM (n = 6, P < 0.05). Asterisk (*) indicates significant difference compared with the control cells, and two asterisks (**) indicate significant difference compared with the cells treated with ADM2 10−8 M.