Figure 1.

Insulin-Increased PAI-1-Luc expression is affected by transfection with expression plasmids for Fox-family transcription factors: GH4 (A), T47D (B), and HEK293 (C) cells were transfected with PAI-1-Luc, human insulin receptor, and β-galactosidase and HEK293 cells (D and E) were transfected with IRS(3×)-Luc, and β-galactosidase as detailed in Materials and Methods. The electroporations also contained 10 μg of an expression vector for the indicated Fox transcription factor or 10 μg of vector control. The cultures were treated with 1 μg/ml insulin or 1 mm forskolin, and the plates were incubated for 20 h. The average relative light units/10 μg protein in control and insulin-treated cultures was determined, adjusted for β-galactosidase expression, and the relative light units from cells incubated with hormones were compared with control levels to determine the fold-stimulation (Fold-Basal). The results are from three separate experiments done in triplicate. WT, Wild type.