Skip to main content
. 2009 Jul 2;23(10):1681–1690. doi: 10.1210/me.2009-0098

Figure 7.

Figure 7

Increasing PTH1R number inhibits A10 cell proliferation. A, A10 cells were transiently transfected with HA-PTH1R and incubated with BrDU for 18 h. Cell nuclei were stained with DAPI (top left) followed by immunostaining with anti-BrDU (top right) and anti-HA (bottom left) antibodies as detailed in Materials and Methods. The overlay picture (bottom right) shows a cell expressing HA-PTH1R, which is negative for BrDU (arrow), and a nontransfected cell positive for BrDU (arrowhead). B, Quantitation of cell proliferation in naive and PTH1R-expressing cells. The number of BrDU-positive cells was counted in five different fields. The white bar shows the percentage of BrDU-positive, untransfected cells ± se; the gray bar shows the percentage of BrDU-positive, HA-PTH1R-expressing cells ± se. *, P < 0.05.