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. 2009 Jun 25;23(10):1624–1633. doi: 10.1210/me.2008-0365

Figure 7.

Figure 7

The prooncogenic phosphorylation event by CK2 is not inhibited by the tumor-suppressive phosphorylation by DNA-PK. A, 22RV1 cells were transfected with pCMV, pCMV-IGFBP-3, pCMV-IGFBP-3/S156A, or pCMV-IGFBP-3/S167A and incubated for 48 h. After 24 h in SF media, cells were incubated with or without 10 μm NU7026 or 100 nm DMAT. Apoptosis was assessed by histone-associated DNA fragmentation ELISA. B, M059K and M059J cells were transfected with pCMV, pCMV-IGFBP3, or pCMV-IGFBP-3/S167A and incubated for 48 h. Apoptosis was assessed as in A after incubation in SF media for 24 h. Results are shown as means ± sem. Significance that mean is different from 1: *, P < 0.05; **, P < 0.01; n = 3.