Table 1.

Fluorescence intensity ratios of mother/daughter kinetochore clusters obtained from FRAP experiments in which an anaphase cell with a photobleached mother kinetochore spot was observed over time until the mother cell budded and completed S phase

	Prebleach	Postbleach	Recovery
Cse4 GFP	0.99 ± 0.04, n = 20	0.03 ± 0.17, n= 15	1.32 ± 0.11, n = 5
Dsn1 GFP	0.99 ± 0.05, n = 20	0.05 ± 0.11, n = 18	0.97 ± 0.10, n = 4
Spc105 GFP	0.99 ± 0.05, n = 20	0.06 ± 0.24, n = 11	1.13 ± 0.14, n = 7
Nuf2 GFP	0.96 ± 0.03, n = 20	0.03 ± 0.32, n = 14	0.55 ± 0.12, n = 5
Ndc80 GFP	1.00 ± 0.04, n = 20	0.07 ± 0.19, n = 17	0.57 ± 0.06, n = 3

The inner kinetochore proteins (Cse4p, Dsn1p, and Spc105p) have an average ratio prebleach of 0.99 ± 0.05, n = 60; postbleach of 0.05 ± 0.14, n = 44; and recovery of 1.15 ± 0.12, n = 16. The outer kinetochore proteins Nuf2p and Ndc80p have an average ratio prebleach of 0.98± 0.04, n = 40; postbleach of 0.06 ± 0.27, n = 31; and recovery of 0.56 ± 0.18, n = 8.