Figure 6.
Models for the disruption of the alphoidtetO HAC kinetochore mediated by KAP1 targeting. (A) The alphoidtetO kinetochore, with H3 and CENP-A containing nucleosomes (blue and orange, respectively). CENP-C is associated with H3-containing nucleosomes (Hori et al., 2008). A microtubule (green) is bound by the KMN complex (Cheeseman et al., 2006) anchored through the CCAN (constitutive centromere-associated network) complex (Cheeseman and Desai, 2008). (B) Model 1, chromatin modifiers evict CENP-A and causing the kinetochore to fall apart. (C) Model 2, chromatin modifiers modify nonchromatin components, causing the kinetochore to fall apart. (D) Model 3, chromatin modifiers alter H3-containing nucleosomes, resulting in dissociation of CENP-C and dissolution of the kinetochore.