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. 2009 Oct 1;20(19):4246–4255. doi: 10.1091/mbc.E09-05-0378

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© 2009 by The American Society for Cell Biology

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Figure 3. — CENP-C is required for CENP-E assembly in Xenopus egg extract. (A) CENP-C–depleted CSF egg extracts fail to assemble CENP-C and -E at centromeres. Xenopus sperm chromatin incubated in mock-depleted extracts (left) and CENP-C–depleted extracts (right) was stained for CENP-A and -C or -E. Bar, 5 μm. (B) Addition of MYC-tagged CENP-C to CENP-C–depleted extracts restores CENP-C to endogenous levels (left). An additional ∼119-kDa results from the in vitro translation reaction used to complement the extract, as seen in the MYC immunoblot (right). (C) Addback of MYC-CENP-C rescues CENP-E kinetochore localization in extracts depleted of endogenous CENP-C. Bar, 5 μm.