Table 2.
Sample | Final concentration in mg/L (and % recovery) after dilution of sample in BNII Diluent or Normal Serum b |
||||||
---|---|---|---|---|---|---|---|
1 in 100 | 1 in 133 | 1 in 200 | 1 in 400 | 1 in 133 | 1 in 200 | 1 in 400 | |
BNII DIL | BNII DIL | BNII DIL | BNII DIL | Normal serum | Normal serum | Normal serum | |
κFLC polyclonal | 171 (100%) |
175 (102%) |
198 (116%) |
197 (115%) |
163 (95%) |
161 (94%) |
148 (87%) |
λFLC polyclonal | 79 (100%) |
81 (102%) |
88 (111%) |
95 (120%) |
81 (102%) |
87 (110%) |
96 (121%) |
κFLC monoclonal | 132 (100%) |
151 (114%) |
168 (127%) |
164 (124%) |
129 (97%) |
131 (100%) |
128 (97%) |
λFLC monoclonal | 278 (100%) |
316 (114%) |
294 (106%) |
286 (103%) |
314 (113%) |
303 (109%) |
312 (112%) |
FLC measured by BNII (Siemens, Germany);
FLC concentration of normal serum was subtracted prior to final calculation.