Fig. 5.

RT-PCR analysis of differentially expressed genes. RT-PCR analysis was carried out for 25 selected transcripts as indicated. The ACTIN gene was used as an internal control. FG531364, FG532117, EX570080, FG536909, and FG538772 were identified as those that were significantly down-regulated in the SAM in comparison to the NM. Negative control consisted of samples subjected to PCR analysis with ACTIN primers to examine the absence of any genomic DNA. Lanes 1, 2, 3, and 4 contain samples from SAM, AM, RAM, and non-meristematic tissues (a mixture of tendril, leaf, stem, and mature root tissues), respectively.