Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Eur J Immunol. 2009 Jan;39(1):216–224. doi: 10.1002/eji.200838475

Figure 1. Diabetic NOD mice have elevated IL-17 and IL-17F expression.

Figure 1

A. mRNA from pancreas of diabetic, 11 weeks, and 2 weeks old mice were analyzed for the expression of IL-17 and IL17F mRNA by real-time RT-PCR. RNA was normalized to β-actin. B and C. Spleen and lymph nodes from 4 week and 1 year old C57BL/6 or NOD diabetic, pre-diabetic (15 to 17 weeks old) and young adults (6 weeks) were activated with PMA and Ionomycin for 5 hours and further analyzed IL-17 and IFN-γ by ICS. B. Plots for young and diabetic NOD mice. Shown are cells gated on CD4+. C. Averages of the percentage of IL-17 or IFN-γ positive cells gated from the CD4+Tcr-β+ cells (n= 6–8 mice per group analyzed in independent experiments). ILN: inguinal lymph nodes; CLN: cervical lymph nodes; MLN: mesenteric lymph nodes and PLN: pancreatic lymph nodes. P values were calculated with Student’s t-test