Photomicrographs of unstained vaginal secretion from mice at (A) pro-estrus, predominantly consisting of nucleated epithelial cells; (B) estrus, with anucleated cornified cells; (C) metestrus, consisting of the three types of cell, leukocytes, cornified, and nucleated epithelial cells; and (D) diestrus, consisting predominantly of leucocytes. Nucleated epithelial cells (N), leucocytes (L), cornified cell (C).