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. Author manuscript; available in PMC: 2010 Jan 13.
Published in final edited form as: Biochemistry. 2009 Jan 13;48(1):87–95. doi: 10.1021/bi801699m

Figure 3.

Figure 3

Optical spectra of Psa CcP for the oxidized (solid) and half-reduced (dash) forms of the enzyme. Oxidized Psa CcP (4 μM) was diluted in 20 mM Hepes, 50 mM NaCl, pH 7.5. The half-reduced state was achieved by incubation in the presence of 20 mM ascorbate with 10 mM CaCl2 for 1 hour at room temperature.