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IVP embryos after high-osmolality treatment and centrifugation. a) After high-osmolality treatment and several-hour culture. b and c) After high-osmolality treatment and culture to blastocyst. d) After warming. e) After removal of the zona pellucida. f) Reexpansion after in vitro culture in BRL cell-conditioned medium. Bar = 50 μm (a, b, d, and e) and 150 μm (c and f).
