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Nuclear transfer embryos after high-osmolality treatment and centrifugation. a and b) After high-osmolality treatment and several-hour culture. c and d) After high-osmolality treatment and culture to blastocyst stage. e) After warming. f) Reexpanded after in vitro culture in BRL cell-conditioned medium. Bar = 50 μm (b, c, e, and f) and 150 μm (a and d).
