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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Nat Med. 2009 Aug 30;15(9):1066–1071. doi: 10.1038/nm.2007

Figure 2.

Figure 2

Irf8−/− mice exhibit severe osteoporosis due to enhanced osteoclast formation. (a) Radiographic analysis of the femur and tibia. Bar, 1 cm. (b) Microcomputed tomography of the femurs of 8-week-old wild-type and Irf8−/− mice (left), and bone morphometric analysis of femurs isolated from8-week-old mice (n = 6 in each group) (right). BV/TV, bone volume per tissue volume; Tb.Th, trabecular bone thickness; Tb.N, trabecular number; N.Nd/TV, number of nodules per tissue volume. **P<0.01; *P<0.05. Bar, 500 µm (c) Histology of femurs from 8-week-old mice in which osteoclasts were stained using TRAP, an enzymatic marker of osteoclasts (left), and histomorphometric analysis of tibias from 8-week-old mice (n=5 in each group) (right). Oc.S/BS, osteoclast surface per bone surface; N.Oc/BS, number of osteoclasts per bone surface. *P<0.05. Bar, 100 µm. (d) Histological photographs of bone formation that show tetracycline-calcein double labeling, which were administered with an interval of 72 h (left), and histomorphometric analysis of the bone formation rate and bone resorption rate in 8-week-old mice (right). BFR/BS, bone formation rate per bone surface; BRs.R, bone resorption rate. **P<0.01. (e) Analysis of IRF8 protein levels in BMs or M-CSF induced BMMs from recipient chimeric mice. (f) Soft-X ray photographs of long bones (tibias and femurs) isolated from chimeric mice. (g) Histological analysis of tibias isolated from chimeric mice (Villanueva bone staining). Bar, 500 µm. (h) Bone morphometric analysis of femurs isolated from chimeric mice. Representative data from one of two independent experiments is shown [n=3 (WT) and 3 (Irf8−/−) in each experiment]. Data are expressed as the mean+SD (n=3). **P<0.01; *P<0.05.

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