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Localisation of GFP-Ran proteins in live human cells. Fluorescence images of live U2OS cells transfected with RFP-histone 2B and (A) GFP-RanWT, (B) GFP-GST, (C) GFP-RanQ69L or (D) GFP-RanT24N showing GFP (green), RFP (red) and merged images. Representative images are shown for individual cells in interphase or during specific phases of mitosis that are readily identified by chromosome condensation state and organisation. Scale bars, 10 μm.
