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Localisation of GFP-RanQ69L to nuclear pore complexes. (A) Merged image of a live RFP-histone 2B/GFP-RanQ69L-transfected U2OS cell exhibiting punctate nuclear rim staining. (B) 4× enlargement of the rectangular section highlighted in (A), showing the regular distribution of NE-associated foci that are most likely to be NPCs (white arrows). (C, D) Merged images of two live RFP-histone/GFP-RanWT-transfected U2OS cells with multiple mini-nuclei. Scale bars, 10 μm (A,C) or 1 μM (B,C).
