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. 2009 Sep 18;10:66. doi: 10.1186/1471-2121-10-66

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Copyright © 2009 Hutchins et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FRAP analysis of GFP-Ran proteins. (A) Example FRAP experiments focused on the nuclei of GFP-Ran^WT- and GFP-Ran^T24N-transfected U2OS cells. Left, fluorescence images of cells; in each panel, top left image shows a cell before bleaching then successive images left to right, top to bottom after bleaching. Scale bars, 10 μm. Right, plots showing recovery of the fluorescence signal in each case and the calculated t_1/2. (B) Differences in FRAP t_1/2values between different forms of Ran, during progression through the cell cycle and in different subcellular compartments: cyt, interphase cytoplasm; nuc, interphase nucleus; meta, metaphase chromatin; telo, telophase chromatin.