HpaR binding to the hpaR-hpaG intergenic region. EMSAs were performed as indicated in Materials and Methods. The DNA probes used were (A) PR-PG and (B) PG and PR. Increasing concentrations of purified HpaR were used. (A) Lane 1, 0 nM; lane 2, 3 nM; lane 3, 10 nM; lane 4, 30 nM; lane 5, 50 nM; lane 6, 75 nM; lane 7, 150 nM; lane 8, 300 nM. (B) Lanes 1 and 9, 0 nM; lane 2, 3 nM; lane 3, 10 nM; lane 4, 30 nM; lane 5, 50 nM; lane 6, 75 nM; lane 7, 100 nM; lane 8, 200 nM; lane 10, 10 nM, lane 11, 30 nM; lane 12, 50 nM; lane 13, 75 nM; lane 14, 100 nM; lane 15, 200 nM; lane 16, 1000 nM.