Table 1. Bacterial strains and plasmids.

Strain or plasmid	Revelant genotype or phenotype	Reference
Escherichia coli K-12
S17-1λpir	Host for pUT-derived plasmids	(11)
MC4100	F– araD319 Δ(argF-lac)U169 relA1flbB5301 deoC1 ptsF25 rbsR	(5)
AFMC	MC4100 Rifr	(12)
MCR14	AFMC derivative, Pr::lacZ, Tcr	This work
MCR141	MCR14 derivative, Pr::lacZ, hpa complete pathway, Kmr, Tcr	This work
MCR142	MCR14 derivative, Pr::lacZ, Pr::hpaR, Kmr, Tcr	This work
Escherichia coli W
W14	W derivative (Δpaa)	(13)
AF15	W14 derivative (ΔlacZ), Rifr	(12)
WPG11	W14 derivative (ΔlacZ, Pg::lacZ), Kmr	(6)
WPR13	W14 derivative (ΔlacZ, Pr::lacZ), Kmr	This work
Plasmids
pUJ9	Promoterless lacZ vector, Apr	(14)
pAJ40	pUC18 derivative containing hpa pathway	(1)
pHCR2	pACYC184 derivative with an EcoRI DNA fragment containing hpaR	(1)
pUTminiTn5-Km	MiniTn5 delivery plasmid, Kmr, Apr	(14)
pUTminiTn5-Tc	MiniTn5 delivery plasmid, Tcr, Apr	(14)
pUC18Not	Identical to pUC18 but with NotI sites flanking MCS	(15)
pUCR1	pUC18Not derivative overexpressing the hpaR gene under Plac	This work
pJCD01	pUC19 derivative, vector for in vitro transcription	(16)
pBF1	pJCD01 derivative containing Pr-Pg promoters	This work
pBM2	pUJ9 derivative, Pr::lacZ, Apr	This work
pPR13	pUTminiTn5-Km derivative, Pr::lacZ, Kmr, Apr	This work
pPR14	pUTminiTn5-Tc derivative, Pr::lacZ, Tcr, Apr	This work
pAJ402	pUTminiTn5-Km derivative containing hpa pathway	(1)
pBA1	pUCNot derivative, Pr::hpaR, Kmr, Apr	This work
pBA2	pUTminiTn5-Km derivative, Pr::hpaR, Kmr, Apr	This work