Table 2.
scFv | Inhibition of invasion (%)1 | Binding to complex antigens2 | Putative mol. weight of recognized antigen (kDa)3 | Sporozoite surface binding4 | ||||
Oocyst extract |
Sporozoite extract |
Merozoite extract |
Reduced | Non-reduced | FACS | IFAT | ||
AA19 | 28 ± 14 | + | - | - | 83-175 | 83-175 | - | - |
AA28 | 36 ± 16 | + | - | - | 100-150 | 100-150 | - | - |
AB09 | 52 ± 15 | + | + | - | n.d. | 15-20 | + | + |
AB21 | 32 ± 7 | + | + | - | n.d. | 15-20 | + | + |
AB28 | 25 ± 3 | + | + | - | Negative | 15-20 | + | + |
AD10 | 36 ± 12 | + | + | - | Negative | 15-20 | + | + |
1 In each experiment, 10 μg bacterially expressed and purified scFv was used. Means and SDs of triplicates are shown.
2 As determined by ELISA.
3 As estimated by comparison with molecular weight markers in Western blot analyses.
4 As determined by IFAT and flow cytometry (FACS).
+, binding; -, no binding; n.d., not determined.