Table 3.
Antigen and species specificity of the selected scFvs with anti-sporozoite invasion activity, as determined by ELISA (E) and Western blot analyses (W).
| scFv | Recombinant antigen | |||||||
| EtSAG1 | EtMic1 | EtMic2 | EtMic3 | EtMic5 | Eimepsin | MZP 5-7 | 19 kDa Ag | |
| AA19 | -(E) | -(W) | -(E) | -(W) | -(W) | -(W) | -(E) | -(E) |
| AA28 | -(E) | -(W) | -(E) | -(W) | -(W) | -(W) | -(E) | -(E) |
| AB09 | +(E, W) | -(W) | -(E) | -(W) | -(W) | -(W) | -(E) | -(E) |
| AB21 | +(E) | n.t. | -(E) | n.t. | n.t. | n.t. | -(E) | -(E) |
| AB28 | +(E, W) | -(W) | -(E) | -(W) | -(W) | -(W) | -(E) | -(E) |
| AD10 | +(E, W) | n.t. | n.t. | n.t. | n.t. | n.t. | n.t. | -(E) |
| scFv | Eimeria species | |||||||
| E. tenella | E. acervulina | E. brunetti | E. papillata | E. nieschulzi | ||||
| AA19 | +(E, W) | +(E, W) | +(E) | +(E) | -(E) | |||
| AA28 | +(E, W) | +(E, W) | +(E, W) | +(E) | -(E) | |||
| AB09 | +(E, W) | -(E, W) | -(E) | -(E) | -(E) | |||
| AB21 | +(E, W) | -(E, W) | -(E) | -(E) | -(E) | |||
| AB28 | +(E, W) | -(E, W) | -(E, W) | -(E) | -(E) | |||
| AD10 | +(E, W) | -(E, W) | -(E) | -(E) | -(E) | |||
+, binding; -, no binding; n.t., not tested.