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Nitrotyrosine in the extracellular matrix (ECM) of human cartilage samples, as determined by fluorescence immunohistochemistry. A, Osteoarthritic cartilage immunostained for 3-nitrotyrosine shows a distinct speckled pattern of labeling in the pericellular ECM as well as intracellular fluorescence. B, Control cartilage shows intracellular staining for 3-nitrotyrosine, but no staining of the ECM. (Original magnification × 100.)
