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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cancer Res. 2009 Jun 23;69(13):5312–5320. doi: 10.1158/0008-5472.CAN-08-3767

Figure 1. Notch signaling pathway activation status in melanocytes and melanoma cells.

Figure 1

A, Immunohistochemical analysis of active Notch1 expression in primary human melanoma lesions. Anti-HMB45 was employed to identify cells of melanocytic origin. Darker staining depicts melanin production whereas active Notch1 and HMB45 are indicated by red staining. Unaffected epidermal and dermal tissue immediately adjacent to the primary melanoma lesion was also photographed for control purposes.

B, Quantitative RT-PCR analysis of Notch1 expression in a panel of melanoma lesions and cell lines. Fold change is reflected against a representative human melanocyte cell line, HEMN.

C, (Top) Microarray analysis of Hes1, Hey1 and Hey2 expression in four laser microdissected melanoma lesions, four melanoma cell lines, and four primary melanocyte cell lines. (Bottom) Microarray analysis of Hes1, Hey1 and Hey2 expression in a panel of human melanoma cell lines.

D, Western blot analysis of activated Notch1 protein in melanocytes and melanoma cells from C.