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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cancer Res. 2009 Jun 23;69(13):5312–5320. doi: 10.1158/0008-5472.CAN-08-3767

Figure 2. Inhibition of Notch signaling suppresses melanoma, but not melanocyte growth in vitro.

Figure 2

A, Expression of DN-MAML protein in melanoma cell line WM 3248 was detected by immunoblotting for the myc-tagged DN-MAML. Effect of DN-MAML expression on Notch1 targets Hes1, Hey1 and Hey2 was determined by quantitative RT-PCR in 2 melanoma cell lines, WM3248 and WM1366.

B, 7 day growth curves of melanocytes and melanoma cells infected with DN-MAML or GFP lentiviruses. * p<0.05 (Student's T-test).

C, Growth inhibition in melanocytes and melanoma cells in the presence of increasing concentrations of a γ-secretase inhibitor. Cell growth was determined by MTT analysis. Results are percentage of growth inhibition compared with untreated controls (adjusted to 0%). * p<0.005 (Student's t-test).