Figure 3. Constitutive activation of the Notch1 pathway enhances melanocyte growth in vitro.
A, (Left) mRNA analysis of Notch1 expression in GFP- and NIC-infected FOM 117 cells by RT-PCR. (Right) Western blot analysis of Notch1 protein expression in GFP and NIC-infected FOM 117 cells.
B, Immunofluorescence analysis of NIC localization in FOM 117 GFP and NIC-infected cells.
C, mRNA expression of Hey1 and Hey2 in FOM 117 GFP and NIC-infected cells as determined by quantitative RT-PCR.
D, ∼ 2 week growth curve of GFP- and NIC-infected FOM 123 and 124 cells. *p<0.005 (Student's T-test).