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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Biochem J. 2008 Jul 15;413(2):315–322. doi: 10.1042/BJ20071559

Figure 2. Effect of S-100 extracts on in vitro translation of specific transcripts.

Figure 2

(A) Cytoplasmic extracts from control (C) or PKC antisense (As)-treated cells were added to in vitro translation reactions in the presence of specific LPL transcripts (left). The bar graph represents the means of three similar experiments, expressed as % of control. *P < 0.01, **P < 0.05 compared with the control. (B) Nucleotide sequence 1512–1812 of the LPL 3′UTR or an irrelevant 3′UTR of 157 bases were cloned 3′ to the luciferase coding sequence. These constructs were used by us in earlier studies [9]. The constructs were linearized and transcribed as described in the Experimental section. The transcripts generated were translated by using a rabbit reticulocyte in vitro translation system in the presence of cytoplasmic extracts from control or PKC antisense-treated cells. The bar graph represents the means of three similar experiments expressed as % of control. **P < 0.05 compared with the control.