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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Biochem J. 2008 Jul 15;413(2):315–322. doi: 10.1042/BJ20071559

Figure 3. Assessment of the binding of cytoplasmic factors in PKCα-depleted cells to sequences on the 3′ UTR of LPL.

Figure 3

Gel shift and RNase protection analyses have been performed to assess the binding of cytoplasmic factors to sequences on the LPL 3′ UTR. [32P]UTP-labelled transcripts corresponding to nt 1512–1663 of LPL mRNA were incubated with 1–5 μg of S-100 extract from control and antisense PKCα-treated adipocytes, followed by the digestion of unprotected RNA with RNase T1. A specific RNaseT1 protected gel shift was obtained consistently only with the transcript 1512–1663 as is illustrated in the Figure. The control cell extract from cells treated with Lipofectin® alone provided a weaker gel shift.