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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Biochem J. 2008 Jul 15;413(2):315–322. doi: 10.1042/BJ20071559

Figure 6. PKCα mRNA depletion is accompanied by activation of PKA.

Figure 6

3T3-F442A adipocytes were transfected with 0.5 μMPKCα antisense (As PKC alpha) oligomers, and PKCα mRNA expression as well as PKA activity was measured at 24, 48 and 72 h after transfection. (A) PKCα mRNA expression was inhibited by 45 ± 10% at 48 h and by 55 ± 10% at 72 h after transfection. (B) PKA activity increased 2-fold by 100 ± 15% at both time points. The results represent the means of at least three experiments done in triplicate. *P < 0.01, **P < 0.05 compared with the untreated control.