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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2008 Feb 29;86(1-4):35–40. doi: 10.1016/j.prostaglandins.2008.02.001

Figure 3.

Figure 3

Effects of EP2A (1 μM) and PGE2 (1 μM) on osteocalcin (OCN) mRNA expression in MSC after 21 days of culture from EP2 receptor WT, Het and KO mice in the presence of NS-398 (0.1 μM). Analysis for mRNA expression was done by real-time PCR using relative quantification (ΔΔCt method). (A) OCN mRNA expression (relative quantification values). (B) Fold change in OCN mRNA expression relative to WT control after pool of 2 independent experiments. Each bar is the mean ± SEM of n = 3–6 samples. aSignificant effect of treatment, p <0.01. bSignificant effect of genotype, p <0.01; cp <0.05.