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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: J Neurochem. 2009 Jan;108(1):231–245. doi: 10.1111/j.1471-4159.2008.05758.x

Fig. 10.

Fig. 10

DCX induces localization of PP1 in the cytosol. (a) Nuclear staining with DAPI (DAPI), immunofluorescence staining of PP1 labeled with FITC (PP1-FITC), kinesin-13 labeled with CY3 (kinesin-CY3), and merged images (Merged) of the nuclear staining, PP1 and Kinesin-13 are indicated in mock (U87Control), DCX transfected U87 (U87DCX), mock (293T Cont.) and DCX transfected HEK 293T (293T DCX) cells. The white arrows indicate the merged images of nuclei, PP1 and Kinesin-13 (the mixture of blue, green and red fluorescence) as nuclear localization of PP1 and kinesin-13 in control U87, 293T and DCX transfected 293T cells. The yellow arrows indicate the merged images of nuclei and kinesin-13 (the mixture of blue and red fluorescence) as nuclear localization of kinesin-13 only in DCX transfected U87 cells. (b) In DCX transfected U87 (U87DCX) cells, nuclear staining with DAPI (DAPI), double immunofluorescence of PP1 labeled with FITC (PP1-FITC) and DCX labeled with CY3 (DCX-CY3), and merged images of PP1 and DCX, and counterstaining with DAPI are shown. The arrows indicate yellow fluorescence (the mixture of green and red) and merged images of PP1 and DCX as localization of DCX and PP1 in the cytosol of DCX transfected U87 cells. Scale bar of images = 20μm.