DCX synthesis and NrbII induces apoptosis. (a) TUNEL assay was performed in control, DCX (DCX), NrbII (NrbII), transiently (DCX+NrbII) transfected U87 and HEK 293T cells and examined under phase contrast (Phase) and fluorescence microscope for TUNEL staining (TUNEL). Scale bar of images = 20μm. (b) Bar graph represents the cells in G2-M phase of cell cycle quantified by FACS analysis. The proliferation assays were performed in control, DCX (DCX), NrbII (NrbII), transiently (DCX+NrbII) transfected U87 (c) and HEK 293T (d) cells.