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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Neuron. 2008 Oct 23;60(2):247–257. doi: 10.1016/j.neuron.2008.10.001

Figure 1. CysC Reduction Increases CatB Activity and Lowers Soluble Aβ Levels.

Figure 1

(A) CatB activities in hippocampal lysates from CST3+/+, CST3+/− or CST3−/− mice (n= 6, ***, P < 0.001, one-way ANOVA with Tukey-Kramer post hoc test).

(B) ELISA measurements of Aβ1–42 or Aβ1-x in the supernatants of primary cortical cultures (CST3+/+ or CST3−/−) infected with hAPPV642I adenovirus. All measurements were normalized to protein concentrations in the cell lysates (n= 4, **, P < 0.01, Mann-Whitney U test. Average Aβ levels in CST3+/+ cultures were arbitrarily set as 1).

(C–E) ELISA measurements of hippocampal levels of soluble Aβ1-x and Aβ1–42 in 2–4-month-old hAPP-J20 mice. Deleting one or both alleles of CST3 significantly reduced levels of soluble Aβ1-x (C) and Aβ1–42 (D). Ablating CysC also reduced Aβ1–42/Aβ1-x ratios significantly (E). (n = 9–21 mice/genotype, ***, P < 0.001; **, P < 0.01, *, P < 0.05, one-way ANOVA with Tukey-Kramer post hoc test). Bars = means ± SEM (A–E).