Skip to main content
. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Neuron. 2008 Oct 23;60(2):247–257. doi: 10.1016/j.neuron.2008.10.001

Figure 2. CysC Ablation in hAPP-J20 Mice Lowers Total Plaque Deposition.

Figure 2

(A and E) Photomicrographs of 3D6 immunostaining (A) or thioflavin S staining (E) in the hippocampus of a 5–8-month-old hAPP/CST3+/+ or hAPP/CST3−/− mouse.

(B, D and F) Quantification of plaque load labeled with 3D6 (B and D) or thioflavin S (F) in 5–8-month-old and 8–10-month-old hAPP/CST3+/+ or hAPP/CST3−/− mice (n= 9–15 mice/genotype, **, P < 0.01, *, P < 0.05, unpaired t test). For total plaque load, three hippocampal (B) or cortical (D) sections per mouse were analyzed to determine the percent area covered by 3D6-immunoreactive material. The number of thioflavin S–positive plaques was counted in five hippocampal sections per mouse (F). Bars represent means ± SEM.

(C) The logarithm-transformed plaque load was regressed against both age (5–10-month, n= 23–25 mice/genotype) and genotype (as a dummy variable) and was fit to the following model: Log (Plaque Load) = (−7.912 + (0.892) * Age + (1.015) * Genotype (Dummy). The age-adjusted plaque load in hAPP/CST3+/+ mice (Genotype dummy = 1) was significantly higher than that in hAPP/CST3−/− mice (Genotype dummy = 0), since the β2 coefficient (1.015) is significantly larger than 0 (t = 6.217, P < 0.001).