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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Methods Mol Biol. 2009;590:265–277. doi: 10.1007/978-1-60327-378-7_17

Fig. 3.

Fig. 3

Analysis of SUMO-1 modification by reconstituted in vitro sumoylation reaction. (A) In vitro translated 35S-labeled HSF2 protein was incubated with HeLa cytosol (as a source of E1), Ubc9, SUMO-1, SUMO-2 or with various combinations of each of these, and then subjected to SDS-PAGE followed by autoradiography. The positions of unmodified and SUMO-modified HSF2 are indicated to the right of the panel. (B) In vitro translated 35S-labeled HSF2 protein was subjected to the in vitro SUMO-1 modification assay using either 6xHis-SUMO-1 or GST-SUMO-1 as the SUMO-1 substrate for the reaction. This figure is from our published work (24), used with permission.